23020 Cyanine5 NHS ester, 5 mg 2730.00

發(fā)表于由

Cyanine5 NHS ester

CY5 NHS ester是用于標(biāo)記多肽、蛋白和寡核苷酸的氨基基團(tuán)的活性染料。在標(biāo)記反應(yīng)中該染料需要少量的有機(jī)共溶劑（如DMF，DMSO）（請(qǐng)參照我們的推薦手冊(cè)以獲得更多幫助信息）。對(duì)于可溶性蛋白、各種各樣的多肽和寡核苷酸而言，這種染料是非常理想而且成本低廉的。該染料在有機(jī)溶劑對(duì)小分子物質(zhì)的標(biāo)記也是非常有效的。對(duì)于更多的精細(xì)目標(biāo)物，如易降解蛋白，如果DMF或DMSO會(huì)對(duì)其有影響，可以考慮water-soluble Cy5 NHS ester（水溶性Cy5 NHS ester染料），因?yàn)樗鼈儾恍枰袡C(jī)溶劑助溶，而且具有和熒光染料類(lèi)似的性質(zhì)。 ?

訂購(gòu)貨號(hào) 產(chǎn)品名稱(chēng)及規(guī)格 價(jià)格（￥）
13020 Cyanine5 NHS ester, 1 mg 1430.00
23020 Cyanine5 NHS ester, 5 mg 2730.00
43020 Cyanine5 NHS ester, 25 mg 5330.00
53020 Cyanine5 NHS ester, 50 mg 9035.00
63020 Cyanine5 NHS ester, 100 mg 15470.00

? ? 在過(guò)去的幾年里，CY5熒光團(tuán)已經(jīng)成為生命科學(xué)研究和診斷領(lǐng)域非常流行的分子標(biāo)記物。這些熒光素的最強(qiáng)發(fā)射光為紅光，在這個(gè)范圍大多數(shù)CCD檢測(cè)器具有最大檢測(cè)靈敏度，而且是生物物質(zhì)低背景區(qū)。染料的顏色是非常強(qiáng)烈的，因此在凝膠電泳中低至1nmol的量都能被肉眼觀測(cè)到。CY5 NHS ester是用于標(biāo)記多肽、蛋白和寡核苷酸的氨基基團(tuán)的活性染料。在標(biāo)記反應(yīng)中該染料需要少量的有機(jī)共溶劑（如DMF，DMSO）（請(qǐng)參照我們的推薦手冊(cè)以獲得更多幫助信息）。對(duì)于可溶性蛋白、各種各樣的多肽和寡核苷酸而言，這種染料是非常理想而且成本低廉的。該染料在有機(jī)溶劑對(duì)小分子物質(zhì)的標(biāo)記也是非常有效的。對(duì)于更多的精細(xì)目標(biāo)物，如易降解蛋白，如果DMF或DMSO會(huì)對(duì)其有影響，可以考慮water-soluble Cy5 NHS ester（水溶性Cy5 NHS ester染料），因?yàn)樗鼈儾恍枰袡C(jī)溶劑助溶，而且具有和熒光染料類(lèi)似的性質(zhì)。 ? CY5熒光團(tuán)可以與很多的儀器如熒光顯微鏡、成像儀、掃描儀、熒光分析儀等相兼容。許多的CY5衍生物——CY5 NHS ester可以替代Alexa Fluor 647和 DyLight 649等活化酯（activated esters）。 ? ? ? 推薦手冊(cè) 氨基分子的NHS酯標(biāo)記 ? Cy? is a trademark of GE Healthcare. General properties ?

Appearance: dark blue powder
Molecular weight: 616.19
Molecular formula: C36H42ClN3O4
CAS number: 1032678-42-4, 350686-88-3
Solubility: good in polar (DMSO, DMF) and chlorinated (DCM, chlroform) organic solvents, low solubility in water
Quality control: NMR 1H (95%) and 13C, TLC, functional testing
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download

Spectral properties ?

Excitation maximum, nm: 646
Extinction coefficient at excitation maximum, Lmol-1cm-1: 250000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.2

 

Product citations

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